Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Detector technology | IMAGE PLATE |
| Collection date | 1992 |
| Detector | FUJI |
| Spacegroup name | P 32 1 2 |
| Unit cell lengths | 169.000, 169.000, 221.000 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 2.800 |
| R-factor | 0.271 * |
| Rwork | 0.271 |
| R-free | 0.35200 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 25.900 * |
| Data reduction software | DENZO |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.800 * |
| Rmerge | 0.130 |
| Number of reflections | 76187 * |
| Completeness [%] | 86.6 * |
| Redundancy | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8.8 * | Jacobo-Molina, A., (1991) Biochemistry, 30, 6351. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | Tris | 50 (mM) | pH8.8 |
| 2 | 1 | reservoir | ammonium sulfate | 32.5 (%sat) | |
| 3 | 1 | drop | protein | 21 (mg/ml) |
