2HLV
Bovine Odorant Binding Protein deswapped triple mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-06-23 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.933 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 83.501, 83.501, 48.964 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.650 |
R-factor | 0.18589 |
Rwork | 0.185 |
R-free | 0.21224 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1g85 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.317 |
Data reduction software | DENZO |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 57.000 | 1.700 |
High resolution limit [Å] | 1.650 | 1.650 |
Number of reflections | 21290 | |
<I/σ(I)> | 5.2 | 2 |
Completeness [%] | 99.9 | 99.8 |
Redundancy | 20 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 26% (W/V) PEG 8000, 200 mM Ammonium Sulfate and 10 mM Na Acetate (pH 4.8), protein at 7.5 mg/ml, VAPOR DIFFUSION, HANGING DROP, temperature 298K |