2HLQ
Crystal Structure of the Extracellular Domain of the Type II BMP Receptor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 113 |
| Detector technology | CCD |
| Collection date | 2006-02-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 35.588, 45.078, 48.162 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.540 - 1.450 |
| R-factor | 0.1923 |
| Rwork | 0.190 |
| R-free | 0.23159 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hlr |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.522 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 27.932 | 32.920 | 1.530 |
| High resolution limit [Å] | 1.450 | 4.590 | 1.450 |
| Rmerge | 0.053 | 0.028 | 0.378 |
| Total number of observations | 1739 | 6411 | |
| Number of reflections | 13746 | ||
| <I/σ(I)> | 9.7 | 19.9 | 1.1 |
| Completeness [%] | 96.7 | 95.9 | 87.1 |
| Redundancy | 3.8 | 3.5 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 290 | 0.1M Na Citrate, 15% PEG1500, 0.2M Mg acetate, pH 6.0, vapor diffusion, hanging drop, temperature 290K |
