2HLP
CRYSTAL STRUCTURE OF THE E267R MUTANT OF A HALOPHILIC MALATE DEHYDROGENASE IN THE APO FORM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LURE BEAMLINE DW32 |
| Synchrotron site | LURE |
| Beamline | DW32 |
| Temperature [K] | 279 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 114.160, 129.980, 123.450 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.000 - 2.590 |
| Rwork | 0.194 |
| R-free | 0.23200 * |
| Structure solution method | OTHER |
| Starting model (for MR) | 1hlp |
| RMSD bond length | 0.009 * |
| RMSD bond angle | 1.500 * |
| Data reduction software | XDS |
| Data scaling software | CCP4 |
| Refinement software | CNS (0.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.000 | 2.750 |
| High resolution limit [Å] | 2.590 | 2.590 |
| Rmerge | 0.086 * | 0.271 |
| Total number of observations | 159628 * | |
| Number of reflections | 26445 | |
| <I/σ(I)> | 7.3 | 2.2 |
| Completeness [%] | 88.5 * | 71.3 * |
| Redundancy | 3.8 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7.6 | 6 * | 1.7M NACL, 20MM TRIS, PH 7.6, 57% MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 14 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 50 (mM) | |
| 3 | 1 | drop | 1.8 (M) | ||
| 4 | 1 | drop | MPD | 58 (%) | |
| 5 | 1 | reservoir | MPD | 60-70 (%(v/v)) |
