2HLC
HL COLLAGENASE STRUCTURE AT 1.7A RESOLUTION
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | LURE BEAMLINE DW32 |
| Synchrotron site | LURE |
| Beamline | DW32 |
| Temperature [K] | 280 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-11 |
| Detector | MARRESEARCH |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 111.300, 111.300, 165.680 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.700 |
| R-factor | 0.195 |
| Rwork | 0.195 |
| R-free | 0.24500 |
| Structure solution method | RIGID BODY |
| Starting model (for MR) | 1hyl |
| RMSD bond length | 0.009 |
| RMSD bond angle | 26.790 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((ROTAVATA)) |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.740 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.047 * | 0.478 * |
| Number of reflections | 54909 | |
| <I/σ(I)> | 11.4 | 1.6 |
| Completeness [%] | 96.3 | 99.6 |
| Redundancy | 4.4 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microdialysis * | 7 | 291 * | or vapor diffusion * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 50 (mM) | |
| 3 | 1 | drop | 60 (mM) | ||
| 4 | 1 | drop | ammonium sulfate | 1.23 (M) |
