2HLC

HL COLLAGENASE STRUCTURE AT 1.7A RESOLUTION

Experimental procedure

Source type	SYNCHROTRON
Source details	LURE BEAMLINE DW32
Synchrotron site	LURE
Beamline	DW32
Temperature [K]	280
Detector technology	IMAGE PLATE
Collection date	1995-11
Detector	MARRESEARCH
Spacegroup name	I 4 2 2
Unit cell lengths	111.300, 111.300, 165.680
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.700
R-factor	0.195
Rwork	0.195
R-free	0.24500
Structure solution method	RIGID BODY
Starting model (for MR)	1hyl
RMSD bond length	0.009
RMSD bond angle	26.790 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((ROTAVATA))
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	1.740
High resolution limit [Å]	1.700	1.700
Rmerge	0.047 *	0.478 *
Number of reflections	54909
<I/σ(I)>	11.4	1.6
Completeness [%]	96.3	99.6
Redundancy	4.4	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *	7	291 *	or vapor diffusion *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop	Tris-HCl	50 (mM)
3	1	drop		60 (mM)
4	1	drop	ammonium sulfate	1.23 (M)