2HL9
SUMO protease Ulp1 with the catalytic cysteine oxidized to a sulfonic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-03-27 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 105.735, 39.448, 55.764 |
| Unit cell angles | 90.00, 112.24, 90.00 |
Refinement procedure
| Resolution | 18.070 - 1.900 |
| Rwork | 0.192 |
| R-free | 0.23700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1euv |
| RMSD bond length | 0.024 |
| RMSD bond angle | 1.914 |
| Data reduction software | CrystalClear |
| Data scaling software | d*TREK (9.2SSI) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.620 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.052 | 0.344 |
| Total number of observations | 10404 | |
| Number of reflections | 19528 | |
| <I/σ(I)> | 18.8 | 4.9 |
| Completeness [%] | 97.7 | 89.8 |
| Redundancy | 7.07 | 5.87 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 30%(v/v) pentaerythritol ethoxylate(15/4 EO/OH), 0.05M ammonium sulfate, 100mM hydrogen peroxide , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
