2HL0
Crystal structure of the editing domain of threonyl-tRNA synthetase from Pyrococcus abyssi in complex with seryl-3'-aminoadenosine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2005-11-02 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 53.022, 77.223, 90.952 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.770 - 1.860 |
R-factor | 0.193 |
Rwork | 0.193 |
R-free | 0.22000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1y2q |
RMSD bond length | 0.006 |
RMSD bond angle | 1.400 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CCP4 ((MOLREP)) |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 1.930 |
High resolution limit [Å] | 1.860 | 1.860 |
Rmerge | 0.063 | 0.236 |
Number of reflections | 15968 | |
<I/σ(I)> | 19.65 | 3.85 |
Completeness [%] | 99.1 | 91.4 |
Redundancy | 4.6 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 30% PEG 8000, 0.2M ammonium sulphate, 0.1M sodium cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |