2HKU
Structural Genomics, the crystal structure of a putative transcriptional regulator from Rhodococcus sp. RHA1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-29 |
| Detector | SBC-3 |
| Wavelength(s) | 0.97883 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 94.400, 123.701, 73.840 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.500 - 2.000 |
| R-factor | 0.21317 |
| Rwork | 0.211 |
| R-free | 0.25912 |
| Structure solution method | SAD |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.131 |
| Data reduction software | HKLSBC |
| Data scaling software | HKL-2000 |
| Phasing software | HKLSBC |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.500 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.091 | 0.500 |
| Number of reflections | 29578 | |
| <I/σ(I)> | 27.3 | 2.94 |
| Completeness [%] | 99.8 | 99.1 |
| Redundancy | 8.5 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 30% PEG2000 MME, 0.2M Ammonium Sulphate, 0.1M Tris, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
