2HKP
SUMO protease Ulp1 with the catalytic cysteine oxidized to a sulfenic acid
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2006-03-27 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.54 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 105.089, 40.328, 55.379 |
Unit cell angles | 90.00, 112.02, 90.00 |
Refinement procedure
Resolution | 51.367 - 2.100 |
Rwork | 0.216 |
R-free | 0.26000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1euv |
RMSD bond length | 0.025 |
RMSD bond angle | 1.970 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 51.367 | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.084 | 0.493 |
Total number of observations | 6418 | |
Number of reflections | 14736 | |
<I/σ(I)> | 5.9 | 1.5 |
Completeness [%] | 99.9 | 99.3 |
Redundancy | 3.1 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 20% w/v PEG 3350, 0.2M NaCl and post-soaked in 50mM DTT for 48 hours, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |