2HKL
Crystal structure of Enterococcus faecium L,D-transpeptidase C442S mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-11 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.979587 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 211.140, 132.660, 70.120 |
| Unit cell angles | 90.00, 89.98, 90.00 |
Refinement procedure
| Resolution | 19.880 - 2.600 |
| R-factor | 0.223 |
| Rwork | 0.216 |
| R-free | 0.24900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zat |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.900 | 2.750 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 53071 | |
| <I/σ(I)> | 12.93 | 2.73 |
| Completeness [%] | 86.4 | 81.9 |
| Redundancy | 1.93 | 1.94 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 295 | 0.2M ammonium sulfate, 15% PEG 2K, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
