2HKF
Crystal structure of the Complex Fab M75- Peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 150 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-06-02 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 1 |
| Unit cell lengths | 40.388, 43.145, 58.023 |
| Unit cell angles | 85.03, 88.47, 85.67 |
Refinement procedure
| Resolution | 28.868 - 2.010 |
| R-factor | 0.17899 |
| Rwork | 0.176 |
| R-free | 0.24233 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.380 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.868 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.049 | 0.191 |
| Number of reflections | 24418 | |
| <I/σ(I)> | 12 | 3.2 |
| Completeness [%] | 93.5 | 87.8 |
| Redundancy | 2.4 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 291 | Tris, PEG 2000, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
