2HK1
Crystal structure of D-psicose 3-epimerase (DPEase) in the presence of D-fructose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 4A |
Synchrotron site | PAL/PLS |
Beamline | 4A |
Temperature [K] | 297 |
Detector technology | CCD |
Collection date | 2005-06-22 |
Detector | BRUKER PROTEUM 300 |
Wavelength(s) | 0.97948 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 103.900, 113.500, 133.800 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.500 - 2.300 |
R-factor | 0.192 |
Rwork | 0.192 |
R-free | 0.23900 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.069 | |
Number of reflections | 69649 | |
<I/σ(I)> | 10.5 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 6.2 | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 14%(w/v) PEG 1000, 0.1M imidazole (pH 8.0), 0.2M calcium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K |