2HJG
The crystal structure of the B. subtilis YphC GTPase in complex with GDP
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX10.1 |
Synchrotron site | SRS |
Beamline | PX10.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-06-27 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.9794, 0.9797, 1.0094 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 62.711, 65.045, 110.610 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.960 - 2.500 |
R-factor | 0.21815 |
Rwork | 0.215 |
R-free | 0.27353 |
Structure solution method | MAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.251 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.650 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.098 | 0.320 |
Number of reflections | 16253 | |
<I/σ(I)> | 17.8 | 5.1 |
Completeness [%] | 99.9 | 99 |
Redundancy | 6.8 | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 290 | 0.2M sodium flouride, 20% (w/v) PEG3350, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K |