2HJ8
Solution NMR structure of the C-terminal domain of the interferon alpha-inducible ISG15 protein from Homo sapiens. Northeast Structural Genomics target HR2873B
Spectrometer
Experimental method: SOLUTION NMR
| Spectrometer ID | Spectrometer maker | Spectrometer model | Spectrometer type | Spectrometer field strength |
| 1 | Bruker | AVANCE | 600 | |
| 2 | Varian | INOVA | 600 |
Experiment
| experiment id | conditions id | solution id | Experiment type |
| 1 | 1 | 1 | 3D_15N-separated_NOESY |
| 2 | 1 | 1 | 3D_13C-separated_NOESY |
| 3 | 1 | 1 | HNHA |
| 4 | 1 | 2 | high resolution 2D CH-HSQC (for stereospecific assignment of Val/Leu methyls) |
| 5 | 1 | 1 | 2D 15N,1H heteronuclear NOE |
| 6 | 1 | 1 | 3D TR backbone expts |
| 7 | 1 | 1 | 3D HCCH-COSY, 3D HCCH-TOCSYs |
NMR Sample
| conditions id | NMR sample pH | NMR sample pressure | NMR sample temperature |
| 1 | 6.5 | ambient | 293 |
Conformers
| Conformers Calculated Total Number | 100 |
| Conformers Submitted Total Number | 20 |
