2HJ1

Crystal structure of a 3D domain-swapped dimer of protein HI0395 from Haemophilus influenzae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2006-04-15
Detector	ADSC QUANTUM 315
Wavelength(s)	0.98
Spacegroup name	P 43 21 2
Unit cell lengths	74.381, 74.381, 117.027
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.310 - 2.100
R-factor	0.216
Rwork	0.215
R-free	0.23100
Structure solution method	SAD
RMSD bond length	0.018
RMSD bond angle	1.799
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELXD
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.065	0.427
Number of reflections	19260
<I/σ(I)>	16.2	4.1
Completeness [%]	97.3	98
Redundancy	9	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	2.0 M Ammonium Sulfate, 0.1 M Bis-Tris, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	2.0 M Ammonium Sulfate, 0.1 M Bis-Tris, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	2.0 M Ammonium Sulfate, 0.1 M Bis-Tris, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K