2HIY
The structure of conserved bacterial protein SP0830 from Streptococcus pneumoniae. (CASP Target)
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-12-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97906, 0.97923 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.252, 108.845, 65.527 |
| Unit cell angles | 90.00, 97.15, 90.00 |
Refinement procedure
| Resolution | 48.280 - 1.400 |
| R-factor | 0.16427 |
| Rwork | 0.163 |
| R-free | 0.19439 |
| Structure solution method | MAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.420 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.280 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Number of reflections | 160902 | |
| <I/σ(I)> | 2 | |
| Completeness [%] | 98.2 | 94.4 |
| Redundancy | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 289 | 0.1M Tris pH8.5, 0.2M MgCl2, 25% PEG 3000, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
