2HIN

Structure of N15 Cro at 1.05 A: an ortholog of lambda Cro with a completely different but equally effective dimerization mechanism

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2006-05-18
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.9795
Spacegroup name	P 1 21 1
Unit cell lengths	25.579, 49.512, 43.768
Unit cell angles	90.00, 91.75, 90.00

Refinement procedure

Resolution	24.750 - 1.050
R-factor	0.16
Rwork	0.160
R-free	0.17300
Structure solution method	ACORN (DDM)
Starting model (for MR)	10-RESIDUE ALPHA-HELIX
RMSD bond length	0.011
RMSD bond angle	1.390
Data reduction software	d*TREK
Data scaling software	d*TREK (9.2SSI)
Phasing software	ACORN
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	25.570	25.570	1.090
High resolution limit [Å]	1.050	2.260	1.050
Rmerge	0.074	0.051	0.406
Total number of observations		24143	17575
Number of reflections	49888
<I/σ(I)>	9.3	24.5	2.1
Completeness [%]	98.0	97	96.1
Redundancy	4.19	4.59	3.63

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	0.1 M Tris, 1.8 M ammonium sulfate, 17.5 mg/mL protein, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K