2HII

heterotrimeric PCNA sliding clamp

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 12.3.1
Synchrotron site	ALS
Beamline	12.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2005-12-15
Detector	ADSC QUANTUM 315
Spacegroup name	P 1
Unit cell lengths	36.519, 87.179, 135.874
Unit cell angles	89.80, 86.36, 78.33

Refinement procedure

Resolution	20.000 - 2.790
R-factor	0.218
Rwork	0.215
R-free	0.28200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.389
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.900
High resolution limit [Å]	2.790	2.790
Rmerge	0.077	0.238
Number of reflections	37657
<I/σ(I)>	12.3
Completeness [%]	93.2	68.4
Redundancy	3.7	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.5	298	18-20% PEG 3350, 150mM ammonium citrate, 100mM Bis-Tris, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K, pH 5.50