2HIB
human formylglycine generating enzyme, C336S mutant, iodide co-crystallization
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-05-15 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5419 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 61.890, 109.110, 43.492 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.830 - 2.000 |
| R-factor | 0.17492 |
| Rwork | 0.172 |
| R-free | 0.23785 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2aij |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.264 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.060 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rmerge | 0.132 | 0.413 |
| Number of reflections | 72271 | |
| <I/σ(I)> | 9 | 1.9 |
| Completeness [%] | 96.8 | |
| Redundancy | 3.6 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | PEG 4000, Calcium iodide, TRIS, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
