2HI0

Crystal structure of putative phosphoglycolate phosphatase (YP_619066.1) from Lactobacillus delbrueckii subsp. bulgaricus ATCC BAA-365 at 1.51 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2006-06-04
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91837, 0.97939
Spacegroup name	P 1 21 1
Unit cell lengths	58.636, 38.695, 103.583
Unit cell angles	90.00, 98.09, 90.00

Refinement procedure

Resolution	47.727 - 1.510
R-factor	0.158
Rwork	0.157
R-free	0.19000
Structure solution method	MAD
RMSD bond length	0.017
RMSD bond angle	1.592
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELXD
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.727		1.560
High resolution limit [Å]	1.510	2.580	1.510
Rmerge	0.101	0.068	0.537
Number of reflections	72271
<I/σ(I)>	8.75	15.7	2.1
Completeness [%]	95.8	99.3	82.6
Redundancy	3.61		2.77

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION,SITTING DROP,NANODROP	6.5	277	0.2M NaOAc, 30.0% PEG-8000, 0.1M Cacodylate pH 6.5, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K