2HHH
Crystal structure of kasugamycin bound to the 30S ribosomal subunit
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-04-20 |
Detector | MARRESEARCH |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 410.440, 410.440, 172.650 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.740 - 3.350 |
R-factor | 0.268 |
Rwork | 0.265 |
R-free | 0.28900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | EMPTY 30S |
RMSD bond length | 0.010 |
RMSD bond angle | 1.400 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 98.400 | 3.470 |
High resolution limit [Å] | 3.350 | 3.350 |
Number of reflections | 205944 | |
<I/σ(I)> | 7.7 | 1.7 |
Completeness [%] | 98.4 | 91.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 300 | MPD, MAGNESIUM CHLORIDE, POTASSIUM CHLORIDE, AMMONIUM CHLORIDE, MES-KOH, pH 6.50, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | MPD | ||
10 | 1 | 2 | AMMONIUM CHLORIDE | ||
11 | 1 | 2 | MES-KOH | ||
12 | 1 | 2 | H2O | ||
2 | 1 | 1 | MAGNESIUM CHLORIDE | ||
3 | 1 | 1 | POTASSIUM CHLORIDE | ||
4 | 1 | 1 | AMMONIUM CHLORIDE | ||
5 | 1 | 1 | MES-KOH | ||
6 | 1 | 1 | H2O | ||
7 | 1 | 2 | MPD | ||
8 | 1 | 2 | MAGNESIUM CHLORIDE | ||
9 | 1 | 2 | POTASSIUM CHLORIDE |