2HHF
X-ray crystal structure of oxidized human mitochondrial branched chain aminotransferase (hBCATm)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2002-12-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.91609 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.123, 104.183, 58.264 |
| Unit cell angles | 90.00, 100.72, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.800 |
| R-factor | 0.283 |
| Rwork | 0.252 |
| R-free | 0.28300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ekf |
| RMSD bond length | 0.014 |
| RMSD bond angle | 2.066 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.077 | 0.483 |
| Number of reflections | 58070 | |
| <I/σ(I)> | 9 | |
| Completeness [%] | 92.0 | 86.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 294 | Peg1500, Hepes, pH 7.0, H2O2, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
