2HGV
N-terminal GAF domain of transcriptional pleiotropic repressor CodY
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-25 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9800 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 34.690, 84.846, 54.929 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.720 - 2.300 |
| R-factor | 0.16804 |
| Rwork | 0.164 |
| R-free | 0.24418 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b18 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.408 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.104 | 0.361 |
| Number of reflections | 7646 | |
| <I/σ(I)> | 13.7 | |
| Completeness [%] | 99.0 | 92.2 |
| Redundancy | 6.1 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | PEG 5000, calcium acetate, valine, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
