2HF0
Bifidobacterium longum bile salt hydrolase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-01-30 |
Wavelength(s) | 1.5148 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 123.981, 123.981, 219.565 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 2.300 |
R-factor | 0.18699 |
Rwork | 0.185 |
R-free | 0.22835 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.029 |
RMSD bond angle | 2.100 |
Phasing software | AMoRE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 107.211 | 2.300 |
High resolution limit [Å] | 2.250 | 2.250 |
Number of reflections | 46662 | |
Completeness [%] | 99 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.1 | 303 | ammonium sulfate 30% w/v, 0.5 M sodium formate, sucrose (1M) 100ml, 40 uL of b-octyl glucosidase (0.50% w/v), pH 7.1, VAPOR DIFFUSION, HANGING DROP, temperature 303K |