2HES

Cytosolic Iron-sulphur Assembly Protein- 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Detector	MARRESEARCH
Wavelength(s)	0.931
Spacegroup name	P 21 21 21
Unit cell lengths	35.211, 70.026, 130.366
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	17.980 - 1.700
R-factor	0.22584
Rwork	0.224
R-free	0.27544
Structure solution method	SAD
RMSD bond length	0.016
RMSD bond angle	1.784
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	17.980	1.744
High resolution limit [Å]	1.700	1.700
Number of reflections	34661
Completeness [%]	98.3	98.34

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	291	22%PEG 4000, 20mM tris-HCl, pH = 8.0 and 100mM CaCl2, VAPOR DIFFUSION, SITTING DROP, temperature 291.0K
1	VAPOR DIFFUSION, SITTING DROP	8	291	22%PEG 4000, 20mM tris-HCl, pH = 8.0 and 100mM CaCl2, VAPOR DIFFUSION, SITTING DROP, temperature 291.0K