2HEI
Crystal structure of human RAB5B in complex with GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-07 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.302, 78.370, 95.501 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.550 |
| Rwork | 0.262 |
| R-free | 0.29360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1n6i |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.488 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (refmac_5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 3.230 | 1.500 |
| Rmerge | 0.058 | 0.037 | 0.679 |
| Number of reflections | 50161 | 5303 | 4938 |
| <I/σ(I)> | 9.2 | ||
| Completeness [%] | 100.0 | ||
| Redundancy | 7 | 6.7 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 291 | 25% P3350, 0.1M NaOAc, pH 4.5, vapor diffusion, sitting drop, temperature 291K |
