2HE7

FERM domain of EPB41L3 (DAL-1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2006-05-22
Detector	ADSC QUANTUM 315
Wavelength(s)	1.07
Spacegroup name	P 65
Unit cell lengths	135.500, 135.500, 49.800
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.340 - 2.000
R-factor	0.188
Rwork	0.187
R-free	0.21700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.021
RMSD bond angle	1.735
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000		2.100
High resolution limit [Å]	2.000	6.000	2.000
Rmerge		0.025	0.561
Number of reflections	35521
<I/σ(I)>	21.13	70.1	4.8
Completeness [%]	99.4	100	98.4
Redundancy	11

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	20% EtOH 0.1 M Tris pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K