2HE2
Crystal structure of the 3rd PDZ domain of human discs large homologue 2, DLG2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-06 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97646 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.155, 56.070, 60.472 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.130 - 1.500 |
| R-factor | 0.12987 |
| Rwork | 0.127 |
| R-free | 0.18688 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Swissmodel based upon the coordinates of pdb entries 1TP5 1tp3 1BFE and 1BE9. |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.324 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 30918 | |
| Completeness [%] | 98.8 | 97.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 25.5% PEG 3350; 0.17M (NH4)2SO4; 15% glycerol , VAPOR DIFFUSION, SITTING DROP, temperature 293K |
