2HDZ

Crystal Structure Analysis of the UBF HMG box5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-08-24
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9363
Spacegroup name	P 65
Unit cell lengths	60.812, 60.812, 63.158
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 2.000
R-factor	0.21953
Rwork	0.217
R-free	0.26700
Structure solution method	SAD
RMSD bond length	0.013
RMSD bond angle	1.446
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE (2.09)
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.030
High resolution limit [Å]	2.000	2.000
Rmerge	0.046	0.185
Number of reflections	8917
<I/σ(I)>	35.6
Completeness [%]	99.3	93.3
Redundancy	11.1	10

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	NaCl, pH 7.5, vapor diffusion, hanging drop, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	NaCl, pH 7.5, vapor diffusion, hanging drop, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	NaCl, pH 7.5, vapor diffusion, hanging drop, temperature 277K