2HDO

Crystal structure of putative phosphoglycolate phosphatase (np_784602.1) from Lactobacillus plantarum at 1.50 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2006-05-07
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979224, 0.978954, 0.918370
Spacegroup name	P 61
Unit cell lengths	86.890, 86.890, 47.190
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.424 - 1.500
R-factor	0.178
Rwork	0.176
R-free	0.20900
Structure solution method	MAD
RMSD bond length	0.014
RMSD bond angle	1.478
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.424		1.550
High resolution limit [Å]	1.500	2.560	1.500
Rmerge	0.143	0.113	0.426
Number of reflections	32658
<I/σ(I)>	7.33	12.8	2.32
Completeness [%]	96.5	98.2	88.8
Redundancy	8.784

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP, NANODROP	4.2	277	0.4M KH2PO3, 1.6M NaH2PO3, 0.1M Phosphate Citrate, pH 4.2, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 277K