2HDD
ENGRAILED HOMEODOMAIN Q50K VARIANT DNA COMPLEX
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 123 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-06 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 127.720, 45.340, 72.470 |
| Unit cell angles | 90.00, 119.53, 90.00 |
Refinement procedure
| Resolution | 6.000 - 1.900 |
| R-factor | 0.205 |
| Rwork | 0.205 |
| R-free | 0.25100 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.460 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.035 | 0.380 |
| Number of reflections | 27136 | |
| Completeness [%] | 94.0 | 94 * |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | COMPLEX WAS CRYSTALLIZED BY HANGING DROP VAPOR DIFFUSION OVER .75M AMOAC AND 1% PEG400; DROP STARTS AT 1M AMOAC., pH 7.00, vapor diffusion - hanging drop |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | AMMONIUM ACETATE | ||
| 3 | 1 | 1 | PEG 400 | ||
| 4 | 1 | 2 | WATER | ||
| 5 | 1 | 2 | PEG 400 | ||
| 6 | 1 | 2 | AMMONIUM ACETATE |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | complex | 10 (mg/ml) | |
| 2 | 1 | drop | ammonium acetate | 1 (M) | |
| 3 | 1 | reservoir | ammonium acetate | 0.73-0.80 (M) | |
| 4 | 1 | reservoir | PEG400 | 1 (%) |
