2HD3
Crystal Structure of the Ethanolamine Utilization Protein EutN from Escherichia coli, NESG Target ER316
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-08 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97922 |
| Spacegroup name | P 1 |
| Unit cell lengths | 49.274, 72.574, 84.496 |
| Unit cell angles | 79.22, 85.43, 81.39 |
Refinement procedure
| Resolution | 27.740 - 2.400 |
| R-factor | 0.234 |
| Rwork | 0.234 |
| R-free | 0.29000 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SnB |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.740 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.105 | 0.458 |
| Number of reflections | 88816 | |
| <I/σ(I)> | 14.9 | 2.1 |
| Completeness [%] | 96.9 | 97.1 |
| Redundancy | 3.9 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 100mM HEPES, 23% PEG 20k, 5 mM DTT, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
