2HCZ
Crystal structure of EXPB1 (Zea m 1), a beta-expansin and group-1 pollen allergen from maize
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-10-01 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 113.408, 44.512, 69.467 |
| Unit cell angles | 90.00, 124.64, 90.00 |
Refinement procedure
| Resolution | 29.630 - 2.750 |
| R-factor | 0.233 |
| Rwork | 0.233 |
| R-free | 0.29000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1n10 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.650 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.850 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.079 | 0.402 |
| Number of reflections | 7013 | |
| <I/σ(I)> | 7.3 | |
| Completeness [%] | 93.1 | 96.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 294 | 15% PEG 4000, 50mM Sodium Acetate and 0.1M Ammonium Sulphate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
