2HCN

Crystal structure of RNA dependent RNA polymerase domain from west nile virus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-3
Synchrotron site	ESRF
Beamline	ID14-3
Temperature [K]	100
Detector technology	CCD
Collection date	2005-11-27
Detector	MAR CCD 165 mm
Wavelength(s)	0.9330
Spacegroup name	P 43
Unit cell lengths	110.064, 110.064, 68.572
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.820 - 2.350
R-factor	0.20962
Rwork	0.207
R-free	0.25944
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	WEST NILE VIRUS RNA DEPENDANT RNA POLYMERASE COORDINATES 2HCS (DOMAIN FROM AMINO ACIDS 317-905)
RMSD bond length	0.021
RMSD bond angle	1.729
Data reduction software	MOSFLM
Phasing software	AMoRE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	69.000	2.480
High resolution limit [Å]	2.350	2.350
Number of reflections	34338
<I/σ(I)>	21.8	1.5
Completeness [%]	99.9	99.7
Redundancy	5.7	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	10% peg 8000, Imid 0.1M, Ca(OAc)2 0.2M, VAPOR DIFFUSION, HANGING DROP, temperature 293K