2HCJ

Trypsin-modified Elongation Factor Tu in complex with tetracycline

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Detector technology	IMAGE PLATE
Collection date	1999-10-04
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 43 21 2
Unit cell lengths	69.110, 69.110, 157.330
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	51.920 - 2.120
Rwork	0.200
R-free	0.23400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Chain A residues 8-39 59-260 264-393 of tm-EF-Tu-MgGDP in space group C2221 1.9A model (unpublished)
RMSD bond length	0.009
RMSD bond angle	1.600
Phasing software	EPMR
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.920	2.180
High resolution limit [Å]	2.120	2.120
Number of reflections	20773
Completeness [%]	93.0	57.5
Redundancy	2.7	1.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP			VAPOR DIFFUSION, SITTING DROP