2HCG
Structure of S65T Y66F GFP variant after cyclization, carbon-carbon bond cleavage, and oxygen incorporation reactions
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-1 |
Synchrotron site | SSRL |
Beamline | BL9-1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2001-12-07 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 0.9706 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 51.132, 62.501, 71.187 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.000 - 1.350 |
R-factor | 0.127 |
Rwork | 0.127 |
R-free | 0.18840 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ema |
RMSD bond length | 0.011 |
RMSD bond angle | 0.029 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.000 | 1.400 |
High resolution limit [Å] | 1.350 | 1.350 |
Rmerge | 0.352 | |
Number of reflections | 50300 | |
<I/σ(I)> | 26.2 | 3.1 |
Completeness [%] | 98.9 | 97.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 19% PEG 4K, 50 mM MgCl2, 50 mM Hepes, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |