2HC8
Structure of the A. fulgidus CopA A-domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 111.273, 29.372, 39.972 |
| Unit cell angles | 90.00, 110.46, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.650 |
| R-factor | 0.205 |
| Rwork | 0.200 |
| R-free | 0.22800 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.402 |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.273 | 1.740 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.083 | 0.389 |
| Total number of observations | 9934 | |
| Number of reflections | 14467 | |
| <I/σ(I)> | 3.9 | 1.7 |
| Completeness [%] | 97.5 | 85 |
| Redundancy | 6.9 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 100mM Tris, 1.8 M ammonium sulfate, 7.5% glycerol, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
