2HBM
Structure of the yeast nuclear exosome component, Rrp6p, reveals an interplay between the active site and the HRDC domain; Protein in complex with Mn, Zn, and UMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | ENRAF-NONIUS FR591 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2006-01-09 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.54179 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 110.577, 110.577, 79.330 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.610 - 2.700 |
R-factor | 0.226 |
Rwork | 0.220 |
R-free | 0.28600 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 2hbj |
RMSD bond length | 0.007 |
RMSD bond angle | 1.340 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.610 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.061 | 0.666 |
Number of reflections | 15522 | 1416 |
<I/σ(I)> | 14.9 | 1.5 |
Completeness [%] | 98.4 | 92.9 |
Redundancy | 4.1 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 12-14% PEG 20000, 0.1M Mes or Hepes, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |