2HBK
Structure of the yeast nuclear exosome component, Rrp6p, reveals an interplay between the active site and the HRDC domain; Protein in complex with Mn
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-10-27 |
Detector | MARRESEARCH |
Wavelength(s) | 1.50 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 110.680, 110.680, 79.729 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 36.220 - 2.250 |
R-factor | 0.228 |
Rwork | 0.222 |
R-free | 0.27900 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 2hbj |
RMSD bond length | 0.007 |
RMSD bond angle | 1.271 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.220 | 2.330 |
High resolution limit [Å] | 2.250 | 2.250 |
Rmerge | 0.040 | 0.341 |
Number of reflections | 27056 | 2637 |
<I/σ(I)> | 19.7 | 4.9 |
Completeness [%] | 99.7 | 98.8 |
Redundancy | 5.6 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 12-14% PEG 20000, 0.1M Mes or Hepes, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |