2HB5

Crystal Structure of the Moloney Murine Leukemia Virus RNase H Domain

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 4
Wavelength(s)	0.98793, 0.97938, 0.97908, 0.96866
Spacegroup name	P 1
Unit cell lengths	32.326, 34.107, 34.802
Unit cell angles	78.21, 69.85, 64.79

Refinement procedure

Resolution	21.660 - 1.590
Rwork	0.196
R-free	0.22090
Structure solution method	MAD
RMSD bond length	0.005
RMSD bond angle	1.261
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SnB
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	21.660	1.660
High resolution limit [Å]	1.590	1.590
Number of reflections	15825
Completeness [%]	93.4	91

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	293	15% PEG 4000, 0.1M ammonium sulfate, 150mM NaCl, 1.25%PEG-MME 550, 1% 2-propanol, 0.5mM zinc sulfate, 5mM MES , pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K