2HAZ
Crystal structure of the first fibronectin domain of human NCAM1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-25 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9766 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 42.760, 53.880, 74.070 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.700 |
| Rwork | 0.212 |
| R-free | 0.28600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | : 1UEM |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.286 |
| Data scaling software | XSCALE |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.790 |
| High resolution limit [Å] | 1.690 | 1.690 |
| Rmerge | 0.095 | 0.487 |
| Number of reflections | 9851 | |
| <I/σ(I)> | 12.88 | 3.1 |
| Completeness [%] | 99.0 | 94.4 |
| Redundancy | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.1 | 298 | 0.3 M Calcium chloride, 17% PEG 3350, 10% PEG 400 (the latter only in the drop), pH 5.1, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
