2HAV
Apo-Human Serum Transferrin (Glycosylated)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 103 |
| Detector technology | CCD |
| Collection date | 2005-08-11 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 88.324, 103.258, 200.362 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.700 |
| R-factor | 0.232 |
| Rwork | 0.232 |
| R-free | 0.29300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | human apo-N-lobe structure (1BP5) and the rabbit holo-C1-subdomain (residues 342 to 424 and 579 to 676 from 1JNF) |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.570 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.068 | |
| Number of reflections | 49871 | |
| <I/σ(I)> | 2 | |
| Completeness [%] | 96.7 | 90.5 |
| Redundancy | 6.6 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 294 | 30 mg/ml protein, 0.2 M ammonium citrate pH 7.0, 20% PEG-3350, 15% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
