2HAK
Catalytic and ubiqutin-associated domains of MARK1/PAR-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-20 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.803 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 110.682, 116.470, 285.748 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.600 |
| R-factor | 0.214 |
| Rwork | 0.212 |
| R-free | 0.29000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zmu |
| RMSD bond length | 0.028 |
| RMSD bond angle | 2.408 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.640 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.082 | 0.473 |
| Number of reflections | 112949 | |
| <I/σ(I)> | 15.4 | 2.6 |
| Completeness [%] | 99.4 | 98.5 |
| Redundancy | 4.8 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 10% PEG 3350, 0.2M ammonium citrate, 0.1M bis-Tris, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
