2HA9
Crystal structure of protein SP0239 from Streptococcus pneumoniae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-03-08 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97915 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 119.906, 139.963, 148.896 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.700 |
R-factor | 0.19754 |
Rwork | 0.194 |
R-free | 0.27127 |
Structure solution method | SAD |
RMSD bond length | 0.035 |
RMSD bond angle | 2.954 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.104 | 0.694 |
Number of reflections | 33065 | |
<I/σ(I)> | 23.14 | 1.82 |
Completeness [%] | 95.5 | 85 |
Redundancy | 6.1 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 289 | 0.2 M NaCl, 0.1M Bis-Tris, 25 % PEG3350, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |