2H9N
WDR5 in complex with monomethylated H3K4 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-05-10 |
| Detector | RIGAKU RAXIS V |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 |
| Unit cell lengths | 46.736, 56.943, 64.795 |
| Unit cell angles | 71.15, 89.31, 66.05 |
Refinement procedure
| Resolution | 60.750 - 2.000 |
| R-factor | 0.22271 |
| Rwork | 0.220 |
| R-free | 0.27944 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.375 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.750 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 36592 | |
| Completeness [%] | 94.6 | 88.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 20% PEG3350, pH 6.5, VAPOR DIFFUSION, HANGING DROP |
