2H9M
WDR5 in complex with unmodified H3K4 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-05-01 |
| Detector | RIGAKU RAXIS V |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 |
| Unit cell lengths | 46.951, 61.375, 64.739 |
| Unit cell angles | 110.34, 91.22, 112.66 |
Refinement procedure
| Resolution | 59.760 - 1.900 |
| R-factor | 0.20656 |
| Rwork | 0.204 |
| R-free | 0.25701 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.164 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.760 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 44230 | |
| Completeness [%] | 91.3 | 73.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 300 | 20% PEG 3350, 0.2M di-Na Tartrate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
