2H9J

Structure of Hen egg white lysozyme soaked with Ni2-Xylylbicyclam

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX14.1
Synchrotron site	SRS
Beamline	PX14.1
Temperature [K]	100
Wavelength(s)	1.488
Spacegroup name	P 43 21 2
Unit cell lengths	77.577, 77.577, 38.355
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.380 - 1.750
R-factor	0.18659
Rwork	0.184
R-free	0.23214
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1yil
RMSD bond length	0.018
RMSD bond angle	1.795
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall
Low resolution limit [Å]	34.380
High resolution limit [Å]	1.750
Number of reflections	11704
Completeness [%]	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	4.5	277	Acetate buffer, NaCl , pH 4.5, VAPOR DIFFUSION, temperature 277K, temperature 277.0K