2H94
Crystal Structure and Mechanism of human Lysine-Specific Demethylase-1
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-10 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0080, 1.2552, 1.2557 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 187.943, 187.943, 108.741 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.900 |
| R-factor | 0.281 |
| Rwork | 0.229 |
| R-free | 0.28100 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.610 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 42195 | |
| Completeness [%] | 98.2 | 88 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 9 % PEG 3350 200 mM Diammonium tartrate 100 mM HEPES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
