2H94

Crystal Structure and Mechanism of human Lysine-Specific Demethylase-1

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2005-10-10
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0080, 1.2552, 1.2557
Spacegroup name	P 61 2 2
Unit cell lengths	187.943, 187.943, 108.741
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.900
R-factor	0.281
Rwork	0.229
R-free	0.28100
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	1.610
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	3.000
High resolution limit [Å]	2.900	2.900
Number of reflections	42195
Completeness [%]	98.2	88

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	9 % PEG 3350 200 mM Diammonium tartrate 100 mM HEPES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K