2H8L
Crystal structure of the bb' fragment of ERp57
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Detector technology | CCD |
| Collection date | 2005-06-10 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9795, 0.9950 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 76.340, 62.415, 99.293 |
| Unit cell angles | 90.00, 98.40, 90.00 |
Refinement procedure
| Resolution | 32.700 - 2.000 |
| R-factor | 0.19709 |
| Rwork | 0.194 |
| R-free | 0.25015 |
| Structure solution method | MAD |
| RMSD bond length | 0.025 |
| RMSD bond angle | 1.932 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 62742 | |
| Completeness [%] | 99.4 | 96.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 30% PEG 3350, 0.1M ammonium sulphate, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
