2H89
Avian Respiratory Complex II with Malonate Bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-07-25 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.1000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 70.520, 84.118, 292.212 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.740 - 2.400 |
R-factor | 0.229 |
Rwork | 0.226 |
R-free | 0.27900 |
Structure solution method | rigid body refinement of previous |
Starting model (for MR) | 1yq3 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.600 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.000 | 2.330 |
High resolution limit [Å] | 2.290 | 2.290 |
Number of reflections | 63148 | |
<I/σ(I)> | 7 | 1.2 |
Completeness [%] | 78.6 | 18.5 |
Redundancy | 2.4 | 1.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 50 G/L PEG-3350, 25 ML/L ISOPROPANOL, 15 ML/L PEG-400 0.05 M NA-HEPES, 0.01 M TRIS-HCL, 0.0005 M MALONATE, 0.0005 M MNCL2, 0.0013 M MGCL2, 0.0015 M NA-AZIDE, 0.00025 M NA-EDTA., pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |